SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae

Anita Minnen, Laetitia Attaiech, Maria Thon, Stephan Gruber, Jan-Willem Veening

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Δsmc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.

Original languageEnglish
Pages (from-to)676-88
Number of pages13
JournalMolecular Microbiology
Volume81
Issue number3
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

Cite this

Minnen, Anita ; Attaiech, Laetitia ; Thon, Maria ; Gruber, Stephan ; Veening, Jan-Willem. / SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae. In: Molecular Microbiology. 2011 ; Vol. 81, No. 3. pp. 676-88.
@article{62b193ad5e7a4fafb96ab4834179e6d2,
title = "SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae",
abstract = "Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Δsmc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.",
keywords = "Bacterial Proteins/genetics, Cell Cycle Proteins/genetics, Chromosome Segregation, Chromosomes, Bacterial/metabolism, DNA-Binding Proteins/metabolism, Gene Deletion, Origin Recognition Complex, Protein Binding, Streptococcus pneumoniae/enzymology",
author = "Anita Minnen and Laetitia Attaiech and Maria Thon and Stephan Gruber and Jan-Willem Veening",
note = "{\circledC} 2011 Blackwell Publishing Ltd.",
year = "2011",
month = "8",
doi = "10.1111/j.1365-2958.2011.07722.x",
language = "English",
volume = "81",
pages = "676--88",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "3",

}

SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae. / Minnen, Anita; Attaiech, Laetitia; Thon, Maria; Gruber, Stephan; Veening, Jan-Willem.

In: Molecular Microbiology, Vol. 81, No. 3, 08.2011, p. 676-88.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae

AU - Minnen, Anita

AU - Attaiech, Laetitia

AU - Thon, Maria

AU - Gruber, Stephan

AU - Veening, Jan-Willem

N1 - © 2011 Blackwell Publishing Ltd.

PY - 2011/8

Y1 - 2011/8

N2 - Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Δsmc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.

AB - Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Δsmc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.

KW - Bacterial Proteins/genetics

KW - Cell Cycle Proteins/genetics

KW - Chromosome Segregation

KW - Chromosomes, Bacterial/metabolism

KW - DNA-Binding Proteins/metabolism

KW - Gene Deletion

KW - Origin Recognition Complex

KW - Protein Binding

KW - Streptococcus pneumoniae/enzymology

U2 - 10.1111/j.1365-2958.2011.07722.x

DO - 10.1111/j.1365-2958.2011.07722.x

M3 - Article

VL - 81

SP - 676

EP - 688

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 3

ER -