Specificity of DC-SIGN for mannose- and fucose-containing glycans

Ellis van Liempt; Christine M C Bank; Padmaja Mehta; Juan Jesús Garciá-Vallejo; Ziad S Kawar; Rudolf Geyer; Richard A Alvarez; Richard D Cummings; Yvette van Kooyk; Irma van Die

doi:10.1016/j.febslet.2006.10.009

Specificity of DC-SIGN for mannose- and fucose-containing glycans

Ellis van Liempt, Christine M C Bank, Padmaja Mehta, Juan Jesús Garciá-Vallejo, Ziad S Kawar, Rudolf Geyer, Richard A Alvarez, Richard D Cummings, Yvette van Kooyk, Irma van Die

Molecular cell biology and Immunology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The dendritic cell specific C-type lectin dendritic cell specific ICAM-3 grabbing non-integrin (DC-SIGN) binds to "self" glycan ligands found on human cells and to "foreign" glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC-SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC-SIGN binds with K(d)<2muM to a neoglycoconjugate in which Galbeta1-4(Fucalpha1-3)GlcNAc (Le(x)) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose-type N-glycans, diantennary N-glycans expressing Le(x) and GalNAcbeta1-4(Fucalpha1-3)GlcNAc (LacdiNAc-fucose), whereas no binding was observed to N-glycans expressing core-fucose linked either alpha1-6 or alpha1-3 to the Asn-linked GlcNAc of N-glycans. These results demonstrate that DC-SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose- and complex-type N-glycans.

Original language	English
Pages (from-to)	6123-31
Number of pages	9
Journal	FEBS Letters
Volume	580
Issue number	26
DOIs	https://doi.org/10.1016/j.febslet.2006.10.009
Publication status	Published - 13 Nov 2006

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10.1016/j.febslet.2006.10.009

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@article{8ad6e7cfba864b4ca5fd50009f74f506,

title = "Specificity of DC-SIGN for mannose- and fucose-containing glycans",

abstract = "The dendritic cell specific C-type lectin dendritic cell specific ICAM-3 grabbing non-integrin (DC-SIGN) binds to {"}self{"} glycan ligands found on human cells and to {"}foreign{"} glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC-SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC-SIGN binds with K(d)<2muM to a neoglycoconjugate in which Galbeta1-4(Fucalpha1-3)GlcNAc (Le(x)) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose-type N-glycans, diantennary N-glycans expressing Le(x) and GalNAcbeta1-4(Fucalpha1-3)GlcNAc (LacdiNAc-fucose), whereas no binding was observed to N-glycans expressing core-fucose linked either alpha1-6 or alpha1-3 to the Asn-linked GlcNAc of N-glycans. These results demonstrate that DC-SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose- and complex-type N-glycans.",

keywords = "Cell Adhesion Molecules/metabolism, Fucose, Humans, Lectins, C-Type/metabolism, Ligands, Mannose, Polysaccharides/chemistry, Protein Array Analysis, Protein Binding, Receptors, Cell Surface/metabolism",

author = "{van Liempt}, Ellis and Bank, {Christine M C} and Padmaja Mehta and Garci{\'a}-Vallejo, {Juan Jes{\'u}s} and Kawar, {Ziad S} and Rudolf Geyer and Alvarez, {Richard A} and Cummings, {Richard D} and Kooyk, {Yvette van} and {van Die}, Irma",

year = "2006",

month = nov,

day = "13",

doi = "10.1016/j.febslet.2006.10.009",

language = "English",

volume = "580",

pages = "6123--31",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "26",

}

TY - JOUR

T1 - Specificity of DC-SIGN for mannose- and fucose-containing glycans

AU - van Liempt, Ellis

AU - Bank, Christine M C

AU - Mehta, Padmaja

AU - Garciá-Vallejo, Juan Jesús

AU - Kawar, Ziad S

AU - Geyer, Rudolf

AU - Alvarez, Richard A

AU - Cummings, Richard D

AU - Kooyk, Yvette van

AU - van Die, Irma

PY - 2006/11/13

Y1 - 2006/11/13

N2 - The dendritic cell specific C-type lectin dendritic cell specific ICAM-3 grabbing non-integrin (DC-SIGN) binds to "self" glycan ligands found on human cells and to "foreign" glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC-SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC-SIGN binds with K(d)<2muM to a neoglycoconjugate in which Galbeta1-4(Fucalpha1-3)GlcNAc (Le(x)) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose-type N-glycans, diantennary N-glycans expressing Le(x) and GalNAcbeta1-4(Fucalpha1-3)GlcNAc (LacdiNAc-fucose), whereas no binding was observed to N-glycans expressing core-fucose linked either alpha1-6 or alpha1-3 to the Asn-linked GlcNAc of N-glycans. These results demonstrate that DC-SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose- and complex-type N-glycans.

AB - The dendritic cell specific C-type lectin dendritic cell specific ICAM-3 grabbing non-integrin (DC-SIGN) binds to "self" glycan ligands found on human cells and to "foreign" glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC-SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC-SIGN binds with K(d)<2muM to a neoglycoconjugate in which Galbeta1-4(Fucalpha1-3)GlcNAc (Le(x)) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose-type N-glycans, diantennary N-glycans expressing Le(x) and GalNAcbeta1-4(Fucalpha1-3)GlcNAc (LacdiNAc-fucose), whereas no binding was observed to N-glycans expressing core-fucose linked either alpha1-6 or alpha1-3 to the Asn-linked GlcNAc of N-glycans. These results demonstrate that DC-SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose- and complex-type N-glycans.

KW - Cell Adhesion Molecules/metabolism

KW - Fucose

KW - Humans

KW - Lectins, C-Type/metabolism

KW - Ligands

KW - Mannose

KW - Polysaccharides/chemistry

KW - Protein Array Analysis

KW - Protein Binding

KW - Receptors, Cell Surface/metabolism

U2 - 10.1016/j.febslet.2006.10.009

DO - 10.1016/j.febslet.2006.10.009

M3 - Article

C2 - 17055489

VL - 580

SP - 6123

EP - 6131

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 26

ER -