Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication

X. Yi, H.I. de Vries, K. Siudeja, A. Rana, W. Lemstra, J.F. Brunsting, R.J.M. Kok, Y.M. Smulders, M. Schaefer, F. Dijk, Y.F. Shang, B.J.L. Eggen, H.H. Kampinga, O.C.M. Sibon

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Abstract

Hydroxyurea, a well-known DNA replication inhibitor, induces cell cycle arrest and intact checkpoint functions are required to survive DNA replication stress induced by this genotoxic agent. Perturbed DNA synthesis also results in elevated levels of DNA damage. It is unclear how organisms prevent accumulation of this type of DNA damage that coincides with hampered DNA synthesis. Here, we report the identification of stonewall (stwl) as a novel hydroxyurea-hypersensitive mutant. We demonstrate that Stwl is required to prevent accumulation of DNA damage induced by hydroxyurea; yet, Stwl is not involved in S/M checkpoint regulation. We show that Stwl is a heterochromatin-associated protein with transcription-repressing capacities. In stwl mutants, levels of trimethylated H3K27 and H3K9 (two hallmarks of silent chromatin) are decreased. Our data provide evidence for a Stwl-dependent epigenetic mechanism that is involved in the maintenance of the normal balance between euchromatin and heterochromatin and that is required to prevent accumulation of DNA damage in the presence of DNA replication stress
Original languageEnglish
Pages (from-to)983-994
Number of pages12
JournalMolecular Biology of the Cell
Volume20
Issue number3
DOIs
Publication statusPublished - 2009

Cite this

Yi, X., de Vries, H. I., Siudeja, K., Rana, A., Lemstra, W., Brunsting, J. F., ... Sibon, O. C. M. (2009). Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication. Molecular Biology of the Cell, 20(3), 983-994. https://doi.org/10.1091/mbc.E08-06-0639
Yi, X. ; de Vries, H.I. ; Siudeja, K. ; Rana, A. ; Lemstra, W. ; Brunsting, J.F. ; Kok, R.J.M. ; Smulders, Y.M. ; Schaefer, M. ; Dijk, F. ; Shang, Y.F. ; Eggen, B.J.L. ; Kampinga, H.H. ; Sibon, O.C.M. / Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 3. pp. 983-994.
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title = "Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication",
abstract = "Hydroxyurea, a well-known DNA replication inhibitor, induces cell cycle arrest and intact checkpoint functions are required to survive DNA replication stress induced by this genotoxic agent. Perturbed DNA synthesis also results in elevated levels of DNA damage. It is unclear how organisms prevent accumulation of this type of DNA damage that coincides with hampered DNA synthesis. Here, we report the identification of stonewall (stwl) as a novel hydroxyurea-hypersensitive mutant. We demonstrate that Stwl is required to prevent accumulation of DNA damage induced by hydroxyurea; yet, Stwl is not involved in S/M checkpoint regulation. We show that Stwl is a heterochromatin-associated protein with transcription-repressing capacities. In stwl mutants, levels of trimethylated H3K27 and H3K9 (two hallmarks of silent chromatin) are decreased. Our data provide evidence for a Stwl-dependent epigenetic mechanism that is involved in the maintenance of the normal balance between euchromatin and heterochromatin and that is required to prevent accumulation of DNA damage in the presence of DNA replication stress",
author = "X. Yi and {de Vries}, H.I. and K. Siudeja and A. Rana and W. Lemstra and J.F. Brunsting and R.J.M. Kok and Y.M. Smulders and M. Schaefer and F. Dijk and Y.F. Shang and B.J.L. Eggen and H.H. Kampinga and O.C.M. Sibon",
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Yi, X, de Vries, HI, Siudeja, K, Rana, A, Lemstra, W, Brunsting, JF, Kok, RJM, Smulders, YM, Schaefer, M, Dijk, F, Shang, YF, Eggen, BJL, Kampinga, HH & Sibon, OCM 2009, 'Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication' Molecular Biology of the Cell, vol. 20, no. 3, pp. 983-994. https://doi.org/10.1091/mbc.E08-06-0639

Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication. / Yi, X.; de Vries, H.I.; Siudeja, K.; Rana, A.; Lemstra, W.; Brunsting, J.F.; Kok, R.J.M.; Smulders, Y.M.; Schaefer, M.; Dijk, F.; Shang, Y.F.; Eggen, B.J.L.; Kampinga, H.H.; Sibon, O.C.M.

In: Molecular Biology of the Cell, Vol. 20, No. 3, 2009, p. 983-994.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication

AU - Yi, X.

AU - de Vries, H.I.

AU - Siudeja, K.

AU - Rana, A.

AU - Lemstra, W.

AU - Brunsting, J.F.

AU - Kok, R.J.M.

AU - Smulders, Y.M.

AU - Schaefer, M.

AU - Dijk, F.

AU - Shang, Y.F.

AU - Eggen, B.J.L.

AU - Kampinga, H.H.

AU - Sibon, O.C.M.

PY - 2009

Y1 - 2009

N2 - Hydroxyurea, a well-known DNA replication inhibitor, induces cell cycle arrest and intact checkpoint functions are required to survive DNA replication stress induced by this genotoxic agent. Perturbed DNA synthesis also results in elevated levels of DNA damage. It is unclear how organisms prevent accumulation of this type of DNA damage that coincides with hampered DNA synthesis. Here, we report the identification of stonewall (stwl) as a novel hydroxyurea-hypersensitive mutant. We demonstrate that Stwl is required to prevent accumulation of DNA damage induced by hydroxyurea; yet, Stwl is not involved in S/M checkpoint regulation. We show that Stwl is a heterochromatin-associated protein with transcription-repressing capacities. In stwl mutants, levels of trimethylated H3K27 and H3K9 (two hallmarks of silent chromatin) are decreased. Our data provide evidence for a Stwl-dependent epigenetic mechanism that is involved in the maintenance of the normal balance between euchromatin and heterochromatin and that is required to prevent accumulation of DNA damage in the presence of DNA replication stress

AB - Hydroxyurea, a well-known DNA replication inhibitor, induces cell cycle arrest and intact checkpoint functions are required to survive DNA replication stress induced by this genotoxic agent. Perturbed DNA synthesis also results in elevated levels of DNA damage. It is unclear how organisms prevent accumulation of this type of DNA damage that coincides with hampered DNA synthesis. Here, we report the identification of stonewall (stwl) as a novel hydroxyurea-hypersensitive mutant. We demonstrate that Stwl is required to prevent accumulation of DNA damage induced by hydroxyurea; yet, Stwl is not involved in S/M checkpoint regulation. We show that Stwl is a heterochromatin-associated protein with transcription-repressing capacities. In stwl mutants, levels of trimethylated H3K27 and H3K9 (two hallmarks of silent chromatin) are decreased. Our data provide evidence for a Stwl-dependent epigenetic mechanism that is involved in the maintenance of the normal balance between euchromatin and heterochromatin and that is required to prevent accumulation of DNA damage in the presence of DNA replication stress

U2 - 10.1091/mbc.E08-06-0639

DO - 10.1091/mbc.E08-06-0639

M3 - Article

VL - 20

SP - 983

EP - 994

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 3

ER -