Synthesis and antibody binding studies of schistosome-derived oligo-α-(1-2)-l-fucosides

Michael R. Harvey, Fabrizio Chiodo, Wouter Noest, Cornelis H. Hokke, Gijsbert A. van der Marel, Jeroen D.C. Codée*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Schistosomiasis is caused by blood-dwelling parasitic trematodes of the genus Schistosoma and is classified by the WHO as the second most socioeconomically devastating parasitic disease, second only to malaria. Schistosoma expresses a complex array of glycans as part of glycoproteins and glycolipids that can be targeted by both the adaptive and the innate part of the immune system. Some of these glycans can be used for diagnostic purposes. A subgroup of schistosome glycans is decorated with unique α-(1-2)-fucosides and it has been shown that these often multi-fucosylated fragments are prime targets for antibodies generated during infection. Since these α-(1-2)-fucosides cannot be obtained in sufficient purity from biological sources, we set out to develop an effective route of synthesis towards α-(1-2)-oligofucosides of varying length. Here we describe the exploration of two different approaches, starting from either end of the fucose chains. The oligosaccharides have been attached to gold nanoparticles and used in an enzyme-linked immunosorbent assay ELISA and a microarray format to probe antibody binding. We show that binding to the oligofucosides of antibodies in sera of infected people depends on the length of the oligofucose chains, with the largest glycans showing most binding.

Original languageEnglish
Article number2246
JournalMolecules
Volume26
Issue number8
DOIs
Publication statusPublished - 2021

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