The blood-brain barrier in cortical multiple sclerosis lesions

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The blood-brain barrier (BBB) is composed mainly of specialized endothelial cells characterized by the presence of intercellular tight junctions. Additionally, perivascular cells, astrocytes, and surrounding basement membranes determine BBB integrity. BBB disruption is an early phenomenon in the formation of new white matter multiple sclerosis (MS) lesions; however, knowledge of the extent of BBB changes in gray matter MS lesions is lacking. Here, we studied several markers for BBB integrity in well-characterized brain tissue of patients with MS. Plasma protein leakage was enhanced in white matter lesions compared with that in normal-appearing white matter, whereas plasma protein leakage was absent in gray matter lesions. White matter lesions showed irregular basement membranes and parenchymal depositions of collagen type IV, whereas purely gray matter lesions lacked basement membrane alterations. Similarly, we observed no evidence for astrogliosis and tight junction changes in cortical MS lesions. Although BBB dysfunction is a common feature of white matter MS lesions, cortical MS lesions lack markers for BBB disruption or astrogliosis. Our data may indicate that BBB breakdown is not a critical event in the formation of gray matter MS lesions.

Original languageEnglish
Pages (from-to)321-8
Number of pages8
JournalJournal of Neuropathology and Experimental Neurology
Volume66
Issue number4
DOIs
Publication statusPublished - Apr 2007

Cite this

@article{b3cbbcd9f7c14daf90fe24ec5295e4dc,
title = "The blood-brain barrier in cortical multiple sclerosis lesions",
abstract = "The blood-brain barrier (BBB) is composed mainly of specialized endothelial cells characterized by the presence of intercellular tight junctions. Additionally, perivascular cells, astrocytes, and surrounding basement membranes determine BBB integrity. BBB disruption is an early phenomenon in the formation of new white matter multiple sclerosis (MS) lesions; however, knowledge of the extent of BBB changes in gray matter MS lesions is lacking. Here, we studied several markers for BBB integrity in well-characterized brain tissue of patients with MS. Plasma protein leakage was enhanced in white matter lesions compared with that in normal-appearing white matter, whereas plasma protein leakage was absent in gray matter lesions. White matter lesions showed irregular basement membranes and parenchymal depositions of collagen type IV, whereas purely gray matter lesions lacked basement membrane alterations. Similarly, we observed no evidence for astrogliosis and tight junction changes in cortical MS lesions. Although BBB dysfunction is a common feature of white matter MS lesions, cortical MS lesions lack markers for BBB disruption or astrogliosis. Our data may indicate that BBB breakdown is not a critical event in the formation of gray matter MS lesions.",
keywords = "Adult, Aged, Basement Membrane/pathology, Blood-Brain Barrier/metabolism, Cerebral Cortex/metabolism, Claudin-5, Collagen Type V/metabolism, Female, Fibrinogen/metabolism, Glial Fibrillary Acidic Protein/metabolism, Humans, Immunoglobulin G/metabolism, Immunohistochemistry, Male, Membrane Proteins, Middle Aged, Multiple Sclerosis/pathology, Tight Junctions/pathology",
author = "{van Horssen}, Jack and Brink, {Bianca P} and {de Vries}, {Helga E} and {van der Valk}, Paul and Lars B{\o}",
year = "2007",
month = "4",
doi = "10.1097/nen.0b013e318040b2de",
language = "English",
volume = "66",
pages = "321--8",
journal = "Journal of Neuropathology and Experimental Neurology",
issn = "0022-3069",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

The blood-brain barrier in cortical multiple sclerosis lesions. / van Horssen, Jack; Brink, Bianca P; de Vries, Helga E; van der Valk, Paul; Bø, Lars.

In: Journal of Neuropathology and Experimental Neurology, Vol. 66, No. 4, 04.2007, p. 321-8.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The blood-brain barrier in cortical multiple sclerosis lesions

AU - van Horssen, Jack

AU - Brink, Bianca P

AU - de Vries, Helga E

AU - van der Valk, Paul

AU - Bø, Lars

PY - 2007/4

Y1 - 2007/4

N2 - The blood-brain barrier (BBB) is composed mainly of specialized endothelial cells characterized by the presence of intercellular tight junctions. Additionally, perivascular cells, astrocytes, and surrounding basement membranes determine BBB integrity. BBB disruption is an early phenomenon in the formation of new white matter multiple sclerosis (MS) lesions; however, knowledge of the extent of BBB changes in gray matter MS lesions is lacking. Here, we studied several markers for BBB integrity in well-characterized brain tissue of patients with MS. Plasma protein leakage was enhanced in white matter lesions compared with that in normal-appearing white matter, whereas plasma protein leakage was absent in gray matter lesions. White matter lesions showed irregular basement membranes and parenchymal depositions of collagen type IV, whereas purely gray matter lesions lacked basement membrane alterations. Similarly, we observed no evidence for astrogliosis and tight junction changes in cortical MS lesions. Although BBB dysfunction is a common feature of white matter MS lesions, cortical MS lesions lack markers for BBB disruption or astrogliosis. Our data may indicate that BBB breakdown is not a critical event in the formation of gray matter MS lesions.

AB - The blood-brain barrier (BBB) is composed mainly of specialized endothelial cells characterized by the presence of intercellular tight junctions. Additionally, perivascular cells, astrocytes, and surrounding basement membranes determine BBB integrity. BBB disruption is an early phenomenon in the formation of new white matter multiple sclerosis (MS) lesions; however, knowledge of the extent of BBB changes in gray matter MS lesions is lacking. Here, we studied several markers for BBB integrity in well-characterized brain tissue of patients with MS. Plasma protein leakage was enhanced in white matter lesions compared with that in normal-appearing white matter, whereas plasma protein leakage was absent in gray matter lesions. White matter lesions showed irregular basement membranes and parenchymal depositions of collagen type IV, whereas purely gray matter lesions lacked basement membrane alterations. Similarly, we observed no evidence for astrogliosis and tight junction changes in cortical MS lesions. Although BBB dysfunction is a common feature of white matter MS lesions, cortical MS lesions lack markers for BBB disruption or astrogliosis. Our data may indicate that BBB breakdown is not a critical event in the formation of gray matter MS lesions.

KW - Adult

KW - Aged

KW - Basement Membrane/pathology

KW - Blood-Brain Barrier/metabolism

KW - Cerebral Cortex/metabolism

KW - Claudin-5

KW - Collagen Type V/metabolism

KW - Female

KW - Fibrinogen/metabolism

KW - Glial Fibrillary Acidic Protein/metabolism

KW - Humans

KW - Immunoglobulin G/metabolism

KW - Immunohistochemistry

KW - Male

KW - Membrane Proteins

KW - Middle Aged

KW - Multiple Sclerosis/pathology

KW - Tight Junctions/pathology

U2 - 10.1097/nen.0b013e318040b2de

DO - 10.1097/nen.0b013e318040b2de

M3 - Article

VL - 66

SP - 321

EP - 328

JO - Journal of Neuropathology and Experimental Neurology

JF - Journal of Neuropathology and Experimental Neurology

SN - 0022-3069

IS - 4

ER -