The cervicovaginal microbiota in women notified for chlamydia trachomatis infection: A case-control study at the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands

Charlotte Van Der Veer, Sylvia M. Bruisten, Jannie J. Van Der Helm, Henry J.C. De Vries, Robin Van Houdt

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background. Increasing evidence suggests that the cervicovaginal microbiota (CVM) plays an important role in acquiring sexually transmitted infections (STIs). Here we study the CVM in a population of women notified by a sex partner for Chlamydia trachomatis infection. Methods. We included 98 women who were contact-traced by C. trachomatis-positive sex partners at the STI outpatient clinic in Amsterdam, the Netherlands, and analyzed their cervicovaginal samples and clinical data. CVMs were characterized by sequencing the V3/V4 region of the 16S ribosomal RNA gene and by hierarchical clustering. Characteristics associating with C. trachomatis infection were examined using bivariable and multivariable logistic regression analysis. Results. The CVM was characterized for 93 women, of whom 52 tested C. trachomatis positive and 41 C. trachomatis negative. We identified 3 major CVM clusters. Clustered CVM predominantly comprised either diverse anaerobic bacteria (n = 39 [42%]), Lactobacillus iners (n = 32 [34%]), or Lactobacillus crispatus (n = 22 [24%]). In multivariable analysis, we found that CVM was significantly associated with C. trachomatis infection (odds ratio [OR], 4.2 [95% confidence interval {CI}, 1.2-15.4] for women with diverse anaerobic CVM and OR, 4.4 [95% CI, 1.3-15.6], for women with L. iners-dominated CVM, compared with women with L. crispatus-dominated CVM), as was younger age (OR, 3.1 [95% CI, 1.1-8.7] for those ≤21 years old) and reporting a steady sex partner (OR, 3.6 [95% CI, 1.4-9.4]). Conclusions. Women who tested positive for Chlamydia trachomatis infection after having been contact-traced by a chlamydiapositive partner were more likely to have CVM dominated by L. iners or by diverse anaerobic bacteria, than by L. crispatus.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalClinical Infectious Diseases
Volume64
Issue number1
DOIs
Publication statusPublished - 2017

Cite this

@article{ac351b1e955d46479b981a4794519719,
title = "The cervicovaginal microbiota in women notified for chlamydia trachomatis infection: A case-control study at the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands",
abstract = "Background. Increasing evidence suggests that the cervicovaginal microbiota (CVM) plays an important role in acquiring sexually transmitted infections (STIs). Here we study the CVM in a population of women notified by a sex partner for Chlamydia trachomatis infection. Methods. We included 98 women who were contact-traced by C. trachomatis-positive sex partners at the STI outpatient clinic in Amsterdam, the Netherlands, and analyzed their cervicovaginal samples and clinical data. CVMs were characterized by sequencing the V3/V4 region of the 16S ribosomal RNA gene and by hierarchical clustering. Characteristics associating with C. trachomatis infection were examined using bivariable and multivariable logistic regression analysis. Results. The CVM was characterized for 93 women, of whom 52 tested C. trachomatis positive and 41 C. trachomatis negative. We identified 3 major CVM clusters. Clustered CVM predominantly comprised either diverse anaerobic bacteria (n = 39 [42{\%}]), Lactobacillus iners (n = 32 [34{\%}]), or Lactobacillus crispatus (n = 22 [24{\%}]). In multivariable analysis, we found that CVM was significantly associated with C. trachomatis infection (odds ratio [OR], 4.2 [95{\%} confidence interval {CI}, 1.2-15.4] for women with diverse anaerobic CVM and OR, 4.4 [95{\%} CI, 1.3-15.6], for women with L. iners-dominated CVM, compared with women with L. crispatus-dominated CVM), as was younger age (OR, 3.1 [95{\%} CI, 1.1-8.7] for those ≤21 years old) and reporting a steady sex partner (OR, 3.6 [95{\%} CI, 1.4-9.4]). Conclusions. Women who tested positive for Chlamydia trachomatis infection after having been contact-traced by a chlamydiapositive partner were more likely to have CVM dominated by L. iners or by diverse anaerobic bacteria, than by L. crispatus.",
keywords = "Cervicovaginal microbiota, Chlamydia trachomatis, Contact tracing",
author = "{Van Der Veer}, Charlotte and Bruisten, {Sylvia M.} and {Van Der Helm}, {Jannie J.} and {De Vries}, {Henry J.C.} and {Van Houdt}, Robin",
year = "2017",
doi = "10.1093/cid/ciw586",
language = "English",
volume = "64",
pages = "24--31",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "1",

}

The cervicovaginal microbiota in women notified for chlamydia trachomatis infection : A case-control study at the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands. / Van Der Veer, Charlotte; Bruisten, Sylvia M.; Van Der Helm, Jannie J.; De Vries, Henry J.C.; Van Houdt, Robin.

In: Clinical Infectious Diseases, Vol. 64, No. 1, 2017, p. 24-31.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The cervicovaginal microbiota in women notified for chlamydia trachomatis infection

T2 - A case-control study at the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands

AU - Van Der Veer, Charlotte

AU - Bruisten, Sylvia M.

AU - Van Der Helm, Jannie J.

AU - De Vries, Henry J.C.

AU - Van Houdt, Robin

PY - 2017

Y1 - 2017

N2 - Background. Increasing evidence suggests that the cervicovaginal microbiota (CVM) plays an important role in acquiring sexually transmitted infections (STIs). Here we study the CVM in a population of women notified by a sex partner for Chlamydia trachomatis infection. Methods. We included 98 women who were contact-traced by C. trachomatis-positive sex partners at the STI outpatient clinic in Amsterdam, the Netherlands, and analyzed their cervicovaginal samples and clinical data. CVMs were characterized by sequencing the V3/V4 region of the 16S ribosomal RNA gene and by hierarchical clustering. Characteristics associating with C. trachomatis infection were examined using bivariable and multivariable logistic regression analysis. Results. The CVM was characterized for 93 women, of whom 52 tested C. trachomatis positive and 41 C. trachomatis negative. We identified 3 major CVM clusters. Clustered CVM predominantly comprised either diverse anaerobic bacteria (n = 39 [42%]), Lactobacillus iners (n = 32 [34%]), or Lactobacillus crispatus (n = 22 [24%]). In multivariable analysis, we found that CVM was significantly associated with C. trachomatis infection (odds ratio [OR], 4.2 [95% confidence interval {CI}, 1.2-15.4] for women with diverse anaerobic CVM and OR, 4.4 [95% CI, 1.3-15.6], for women with L. iners-dominated CVM, compared with women with L. crispatus-dominated CVM), as was younger age (OR, 3.1 [95% CI, 1.1-8.7] for those ≤21 years old) and reporting a steady sex partner (OR, 3.6 [95% CI, 1.4-9.4]). Conclusions. Women who tested positive for Chlamydia trachomatis infection after having been contact-traced by a chlamydiapositive partner were more likely to have CVM dominated by L. iners or by diverse anaerobic bacteria, than by L. crispatus.

AB - Background. Increasing evidence suggests that the cervicovaginal microbiota (CVM) plays an important role in acquiring sexually transmitted infections (STIs). Here we study the CVM in a population of women notified by a sex partner for Chlamydia trachomatis infection. Methods. We included 98 women who were contact-traced by C. trachomatis-positive sex partners at the STI outpatient clinic in Amsterdam, the Netherlands, and analyzed their cervicovaginal samples and clinical data. CVMs were characterized by sequencing the V3/V4 region of the 16S ribosomal RNA gene and by hierarchical clustering. Characteristics associating with C. trachomatis infection were examined using bivariable and multivariable logistic regression analysis. Results. The CVM was characterized for 93 women, of whom 52 tested C. trachomatis positive and 41 C. trachomatis negative. We identified 3 major CVM clusters. Clustered CVM predominantly comprised either diverse anaerobic bacteria (n = 39 [42%]), Lactobacillus iners (n = 32 [34%]), or Lactobacillus crispatus (n = 22 [24%]). In multivariable analysis, we found that CVM was significantly associated with C. trachomatis infection (odds ratio [OR], 4.2 [95% confidence interval {CI}, 1.2-15.4] for women with diverse anaerobic CVM and OR, 4.4 [95% CI, 1.3-15.6], for women with L. iners-dominated CVM, compared with women with L. crispatus-dominated CVM), as was younger age (OR, 3.1 [95% CI, 1.1-8.7] for those ≤21 years old) and reporting a steady sex partner (OR, 3.6 [95% CI, 1.4-9.4]). Conclusions. Women who tested positive for Chlamydia trachomatis infection after having been contact-traced by a chlamydiapositive partner were more likely to have CVM dominated by L. iners or by diverse anaerobic bacteria, than by L. crispatus.

KW - Cervicovaginal microbiota

KW - Chlamydia trachomatis

KW - Contact tracing

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U2 - 10.1093/cid/ciw586

DO - 10.1093/cid/ciw586

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