The contribution of MIB 1 in the accurate grading of vulvar intraepithelial neoplasia

M. Van Beurden*, A. J.M. De Craen, H. C.W. De Vet, J. L.G. Blaauwgeers, P. Drillenburg, M. P.W. Gallee, N. W. De Kraker, F. B. Lammes, F. J.W. Ten Kate

*Corresponding author for this work

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Abstract

Aim - To determine the interobserver variation in scoring presence and grade of vulvar intraepithelial neoplasia (VIN) in haematoxylin/eosin (H/E) slides, MIB 1 slides, and the combined use of H/E and MIB 1 slides. Methods - 10 slides were stained with H/E and MIB 1 with each of the following diagnoses: normal vulvar skin, VIN 1, VIN 2, and VIN 3. Six observers first scored the H/E slides separately from the MIB 1 slides and second the combined H/E and MIB 1 slides. Results - Unweighted group κ for MIB 1 was 0.62 and the weighted group κ was 0.91. This was significantly better than the unweighted group κ for H/E slides (0.47, p = 0.023) as well as the weighted group κ for H/E slides (0.82, p = 0.014). There was no improvement by the combined use of H/E and MIB 1 slides. VIN 2 is far less confused with VIN 3 in the combined use of H/E and MIB 1 slides (9%) than in H/E slides (38%) (p = 0.007). There is a tendency to grade VIN in a two tailed grading system rather than a three tailed grading system, which became more apparent with the combined use of H/E and MIB 1 slides. Conclusions - The interobserver variation with sole use of MIB 1 is better than with the use of H/E stain in VIN. The use of MIB 1 in grading VIN diminishes confusion between VIN 2 and VIN 3 fourfold. A two tailed grading system for VIN seems already to work in daily practice.

Original languageEnglish
Pages (from-to)820-824
Number of pages5
JournalJournal of Clinical Pathology
Volume52
Issue number11
DOIs
Publication statusPublished - 1 Jan 1999

Cite this

Van Beurden, M., De Craen, A. J. M., De Vet, H. C. W., Blaauwgeers, J. L. G., Drillenburg, P., Gallee, M. P. W., ... Ten Kate, F. J. W. (1999). The contribution of MIB 1 in the accurate grading of vulvar intraepithelial neoplasia. Journal of Clinical Pathology, 52(11), 820-824. https://doi.org/10.1136/jcp.52.11.820