The functional activity of high endothelial venules: a role for the subcapsular sinus macrophages in the lymph node

R E Mebius, J Bauer, A J Twisk, J Brevé, G Kraal

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Adherence of lymphocytes to high endothelial venules (HEV3) is the first step in normal lymphocyte emigration and recirculation. At sites of chronic inflammation, venules often become high-walled and may also be a site for leukocytes to leave the bloodstream. The immunologic and inflammatory mediators, responsible for these effects on endothelial cells, may be important for the maintenance and function of HEV in physiological conditions. It is reported here that the morphological and functional aspects of HEV can be studied by organ cultures of lymph nodes (LN). At 24 h of culture, the appearance of the node was still quite normal, whereas the HEV became flat-walled, with a 45-50% reduction in the capacity to bind lymphocytes. This decrease in function of HEV could be reduced when LN were cultured in the presence of lipopolysaccharides (LPS). The effect of LPS on the function of HEV was presumably mediated by macrophages in the subcapsular sinus, because HEV in LN, which were depleted of subcapsular sinus and medullary macrophages previous to culture, could not be stimulated by addition of LPS to the cultures.

Original languageEnglish
Pages (from-to)277-91
Number of pages15
JournalImmunobiology
Volume182
Issue number3-4
Publication statusPublished - Jun 1991

Cite this

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title = "The functional activity of high endothelial venules: a role for the subcapsular sinus macrophages in the lymph node",
abstract = "Adherence of lymphocytes to high endothelial venules (HEV3) is the first step in normal lymphocyte emigration and recirculation. At sites of chronic inflammation, venules often become high-walled and may also be a site for leukocytes to leave the bloodstream. The immunologic and inflammatory mediators, responsible for these effects on endothelial cells, may be important for the maintenance and function of HEV in physiological conditions. It is reported here that the morphological and functional aspects of HEV can be studied by organ cultures of lymph nodes (LN). At 24 h of culture, the appearance of the node was still quite normal, whereas the HEV became flat-walled, with a 45-50{\%} reduction in the capacity to bind lymphocytes. This decrease in function of HEV could be reduced when LN were cultured in the presence of lipopolysaccharides (LPS). The effect of LPS on the function of HEV was presumably mediated by macrophages in the subcapsular sinus, because HEV in LN, which were depleted of subcapsular sinus and medullary macrophages previous to culture, could not be stimulated by addition of LPS to the cultures.",
keywords = "Animals, Clodronic Acid/pharmacology, Cytokines/pharmacology, Endothelium/immunology, Immunohistochemistry, Lipopolysaccharides/pharmacology, Lymph Nodes/immunology, Lymphocytes/immunology, Macrophage Activation, Macrophages/immunology, Mice, Mice, Inbred Strains, Organ Culture Techniques",
author = "Mebius, {R E} and J Bauer and Twisk, {A J} and J Brev{\'e} and G Kraal",
year = "1991",
month = "6",
language = "English",
volume = "182",
pages = "277--91",
journal = "Immunobiology",
issn = "0171-2985",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "3-4",

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The functional activity of high endothelial venules : a role for the subcapsular sinus macrophages in the lymph node. / Mebius, R E; Bauer, J; Twisk, A J; Brevé, J; Kraal, G.

In: Immunobiology, Vol. 182, No. 3-4, 06.1991, p. 277-91.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The functional activity of high endothelial venules

T2 - a role for the subcapsular sinus macrophages in the lymph node

AU - Mebius, R E

AU - Bauer, J

AU - Twisk, A J

AU - Brevé, J

AU - Kraal, G

PY - 1991/6

Y1 - 1991/6

N2 - Adherence of lymphocytes to high endothelial venules (HEV3) is the first step in normal lymphocyte emigration and recirculation. At sites of chronic inflammation, venules often become high-walled and may also be a site for leukocytes to leave the bloodstream. The immunologic and inflammatory mediators, responsible for these effects on endothelial cells, may be important for the maintenance and function of HEV in physiological conditions. It is reported here that the morphological and functional aspects of HEV can be studied by organ cultures of lymph nodes (LN). At 24 h of culture, the appearance of the node was still quite normal, whereas the HEV became flat-walled, with a 45-50% reduction in the capacity to bind lymphocytes. This decrease in function of HEV could be reduced when LN were cultured in the presence of lipopolysaccharides (LPS). The effect of LPS on the function of HEV was presumably mediated by macrophages in the subcapsular sinus, because HEV in LN, which were depleted of subcapsular sinus and medullary macrophages previous to culture, could not be stimulated by addition of LPS to the cultures.

AB - Adherence of lymphocytes to high endothelial venules (HEV3) is the first step in normal lymphocyte emigration and recirculation. At sites of chronic inflammation, venules often become high-walled and may also be a site for leukocytes to leave the bloodstream. The immunologic and inflammatory mediators, responsible for these effects on endothelial cells, may be important for the maintenance and function of HEV in physiological conditions. It is reported here that the morphological and functional aspects of HEV can be studied by organ cultures of lymph nodes (LN). At 24 h of culture, the appearance of the node was still quite normal, whereas the HEV became flat-walled, with a 45-50% reduction in the capacity to bind lymphocytes. This decrease in function of HEV could be reduced when LN were cultured in the presence of lipopolysaccharides (LPS). The effect of LPS on the function of HEV was presumably mediated by macrophages in the subcapsular sinus, because HEV in LN, which were depleted of subcapsular sinus and medullary macrophages previous to culture, could not be stimulated by addition of LPS to the cultures.

KW - Animals

KW - Clodronic Acid/pharmacology

KW - Cytokines/pharmacology

KW - Endothelium/immunology

KW - Immunohistochemistry

KW - Lipopolysaccharides/pharmacology

KW - Lymph Nodes/immunology

KW - Lymphocytes/immunology

KW - Macrophage Activation

KW - Macrophages/immunology

KW - Mice

KW - Mice, Inbred Strains

KW - Organ Culture Techniques

M3 - Article

VL - 182

SP - 277

EP - 291

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 3-4

ER -