The influence of cytokines on the integrity of the blood-brain barrier in vitro

Helga E. De Vries*, Margret C.M. Blom-Roosemalen, Marijke Van Oosten, Albert G. De Boer, Theo J.C. Van Berkel, Douwe D. Breimer, Johan Kuiper

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalJournal of Neuroimmunology
Issue number1
Publication statusPublished - 1996

Cite this