The influence of nifedipine and mioflazine on mitochondrial calcium overload in normoxic and ischaemic guinea-pig hearts

Jacqueline G. Hugtenburg*, Mario J.D. Van Voorst, Jan Van Marle, Marie Jeanne Mathy, Jan Jacob Beckeringh, Henk A. Van Veen, Maarten G.C. Hendriks, Pieter A. Van Zwieten

*Corresponding author for this work

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The influence of nifedipine (20 nM) and mioflazine (300 nM), i.e. concentrations inducing a 60-70% recovery of cardiac function during reperfusion of globally ischaemic guinea-pig working beans, on the mitochondrial calcium content was investigated in normoxic, globally ischaemic and reperfused globally ischaemic guinea-pig working hearts. Mitochondrial calcium was determined electronmicroscopically with the oxalate-pyroantimonate method. In normoxic hearts both nifedipine and mioflazine reduced the mitochondrial calcium content. Global ischaemia for 45 min and subsequent reperfusion for 25 min resulted in a pronounced mitochondrial calcium overload and damage to the cellular structure. In ischaemic and in reperfusion hearts the drugs maintained mitochondrial calcium at pre-ischaemic levels and decreased the damage to the cellular structure.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalEuropean Journal of Pharmacology
Issue number1
Publication statusPublished - 13 Mar 1990

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