The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion

Bart A Westerman, Ankie Poutsma, Kei Maruyama, Henry F J Schrijnemakers, Inge J van Wijk, Cees B M Oudejans

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

During early human pregnancy, extravillous trophoblast cells invade the maternal tissue of the uterus in a way similar to invasion by cancer cells. However, the process of trophoblast invasion is regulated in a time and place restricted way, in contrast to cancer invasion. We screened first trimester placental tissue enriched by extravillous invasive trophoblasts for the expression of proneural basic helix-loop-helix (bHLH) transcription factors, which are important controllers of cell fate. Surprisingly, the presence of NEUROD1, NEUROD2 and ATH2 transcripts was found by reverse transcriptase polymerase chain reaction (RT-PCR) analysis in first trimester placentabed. Of these genes, the proneural genes NEUROD1 and NEUROD2 are expressed in different subsets of invasive trophoblasts. NEUROD1 expression is found in interstitial and endovascular invasive cells, while NEUROD2 expression is observed mainly in endovascular invasive cells, respectively. These data suggest that in addition to the involvement of proneural genes in neuron, neurendocrine and pancreas differentiation, these genes are involved in trophoblast differentation during progression of invasion.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalMechanisms of development
Volume113
Issue number1
Publication statusPublished - Apr 2002

Cite this

Westerman, Bart A ; Poutsma, Ankie ; Maruyama, Kei ; Schrijnemakers, Henry F J ; van Wijk, Inge J ; Oudejans, Cees B M. / The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion. In: Mechanisms of development. 2002 ; Vol. 113, No. 1. pp. 85-90.
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title = "The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion",
abstract = "During early human pregnancy, extravillous trophoblast cells invade the maternal tissue of the uterus in a way similar to invasion by cancer cells. However, the process of trophoblast invasion is regulated in a time and place restricted way, in contrast to cancer invasion. We screened first trimester placental tissue enriched by extravillous invasive trophoblasts for the expression of proneural basic helix-loop-helix (bHLH) transcription factors, which are important controllers of cell fate. Surprisingly, the presence of NEUROD1, NEUROD2 and ATH2 transcripts was found by reverse transcriptase polymerase chain reaction (RT-PCR) analysis in first trimester placentabed. Of these genes, the proneural genes NEUROD1 and NEUROD2 are expressed in different subsets of invasive trophoblasts. NEUROD1 expression is found in interstitial and endovascular invasive cells, while NEUROD2 expression is observed mainly in endovascular invasive cells, respectively. These data suggest that in addition to the involvement of proneural genes in neuron, neurendocrine and pancreas differentiation, these genes are involved in trophoblast differentation during progression of invasion.",
keywords = "Annexins/biosynthesis, Basic Helix-Loop-Helix Transcription Factors, Caenorhabditis elegans Proteins, Cell Differentiation, Cell Lineage, DNA-Binding Proteins/biosynthesis, Female, Gene Expression Regulation, Developmental, Gestational Age, Helminth Proteins/biosynthesis, Humans, Immunohistochemistry, Male, Models, Biological, Multigene Family, Neurons/cytology, Neuropeptides/biosynthesis, Placenta/metabolism, Protein Structure, Tertiary, RNA, Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Trans-Activators/biosynthesis, Transcription Factors/biosynthesis, Trophoblasts/metabolism",
author = "Westerman, {Bart A} and Ankie Poutsma and Kei Maruyama and Schrijnemakers, {Henry F J} and {van Wijk}, {Inge J} and Oudejans, {Cees B M}",
year = "2002",
month = "4",
language = "English",
volume = "113",
pages = "85--90",
journal = "Mechanisms of development",
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Westerman, BA, Poutsma, A, Maruyama, K, Schrijnemakers, HFJ, van Wijk, IJ & Oudejans, CBM 2002, 'The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion' Mechanisms of development, vol. 113, no. 1, pp. 85-90.

The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion. / Westerman, Bart A; Poutsma, Ankie; Maruyama, Kei; Schrijnemakers, Henry F J; van Wijk, Inge J; Oudejans, Cees B M.

In: Mechanisms of development, Vol. 113, No. 1, 04.2002, p. 85-90.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion

AU - Westerman, Bart A

AU - Poutsma, Ankie

AU - Maruyama, Kei

AU - Schrijnemakers, Henry F J

AU - van Wijk, Inge J

AU - Oudejans, Cees B M

PY - 2002/4

Y1 - 2002/4

N2 - During early human pregnancy, extravillous trophoblast cells invade the maternal tissue of the uterus in a way similar to invasion by cancer cells. However, the process of trophoblast invasion is regulated in a time and place restricted way, in contrast to cancer invasion. We screened first trimester placental tissue enriched by extravillous invasive trophoblasts for the expression of proneural basic helix-loop-helix (bHLH) transcription factors, which are important controllers of cell fate. Surprisingly, the presence of NEUROD1, NEUROD2 and ATH2 transcripts was found by reverse transcriptase polymerase chain reaction (RT-PCR) analysis in first trimester placentabed. Of these genes, the proneural genes NEUROD1 and NEUROD2 are expressed in different subsets of invasive trophoblasts. NEUROD1 expression is found in interstitial and endovascular invasive cells, while NEUROD2 expression is observed mainly in endovascular invasive cells, respectively. These data suggest that in addition to the involvement of proneural genes in neuron, neurendocrine and pancreas differentiation, these genes are involved in trophoblast differentation during progression of invasion.

AB - During early human pregnancy, extravillous trophoblast cells invade the maternal tissue of the uterus in a way similar to invasion by cancer cells. However, the process of trophoblast invasion is regulated in a time and place restricted way, in contrast to cancer invasion. We screened first trimester placental tissue enriched by extravillous invasive trophoblasts for the expression of proneural basic helix-loop-helix (bHLH) transcription factors, which are important controllers of cell fate. Surprisingly, the presence of NEUROD1, NEUROD2 and ATH2 transcripts was found by reverse transcriptase polymerase chain reaction (RT-PCR) analysis in first trimester placentabed. Of these genes, the proneural genes NEUROD1 and NEUROD2 are expressed in different subsets of invasive trophoblasts. NEUROD1 expression is found in interstitial and endovascular invasive cells, while NEUROD2 expression is observed mainly in endovascular invasive cells, respectively. These data suggest that in addition to the involvement of proneural genes in neuron, neurendocrine and pancreas differentiation, these genes are involved in trophoblast differentation during progression of invasion.

KW - Annexins/biosynthesis

KW - Basic Helix-Loop-Helix Transcription Factors

KW - Caenorhabditis elegans Proteins

KW - Cell Differentiation

KW - Cell Lineage

KW - DNA-Binding Proteins/biosynthesis

KW - Female

KW - Gene Expression Regulation, Developmental

KW - Gestational Age

KW - Helminth Proteins/biosynthesis

KW - Humans

KW - Immunohistochemistry

KW - Male

KW - Models, Biological

KW - Multigene Family

KW - Neurons/cytology

KW - Neuropeptides/biosynthesis

KW - Placenta/metabolism

KW - Protein Structure, Tertiary

KW - RNA, Messenger/metabolism

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Trans-Activators/biosynthesis

KW - Transcription Factors/biosynthesis

KW - Trophoblasts/metabolism

M3 - Article

VL - 113

SP - 85

EP - 90

JO - Mechanisms of development

JF - Mechanisms of development

SN - 0925-4773

IS - 1

ER -