The role of cyclo-oxygenase 1 and 2 activity in prostaglandin E2 secretion by cultured human adult microglia: Implications for Alzheimer's disease

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Abstract

Microglial cyclo-oxygenase (COX) expression is considered to be important in the pathogenesis of Alzheimer's disease (AD) and, therefore, constitutes a key target for therapeutic intervention. We investigated the influence of AD plaque associated factors on COX-1 and COX-2 expression and activity in adult human microglial cells in vitro. COX-2 immunoreactivity and mRNA were induced by lipopolysaccharide (LPS), not by AD plaque associated cytokines interleukin (IL)-1α, IL-1β, IL-6, tumor necrosis factor (TNF)-α, or amyloid (A)β1-42. To assess functional COX activity, the release of PGE2 into the culture medium was determined. LPS and also arachidonic acid (AA) dose-dependently stimulated PGE2 release. The effects of AA are independent from induction of COX mRNA expression, or of de novo protein synthesis. No effects of either plaque-associated cytokines or Aβ1-42 on PGE2 secretion were seen, even when cells were co-stimulated with AA, to provide enough substrate. COX isotype selective inhibitors were used to discern relative contributions of COX-1 and COX-2 activities to microglial PGE2 secretion. COX-2 and in part COX-1-selective inhibitors inhibited LPS-induced PGE2 secretion, whereas the AA-induced PGE2 secretion was reduced by COX-1-selective inhibitors only. Apparently, adult human microglia in vitro (1) constitutively express COX-1, and (2) do not express COX-2 upon exposure to either Aβ or plaque associated cytokines. In the light of microglial COX activity as a potential therapeutical target in AD, the data presented in this study suggest that classical NSAIDs, rather than selective COX-2 inhibitors, are more potent in reducing microglial prostaglandin secretion.

Original languageEnglish
Pages (from-to)218-226
Number of pages9
JournalBrain Research
Volume951
Issue number2
DOIs
Publication statusPublished - 4 Oct 2002

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