The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization

W E Fibbe, J F Pruijt, Y van Kooyk, C G Figdor, G Opdenakker, R Willemze

Research output: Contribution to journalReview articleAcademicpeer-review

Abstract

The chemokine interleukin-8 (IL-8) is a potent chemoattractant and activator of neutrophils. Upon systemic injection, IL-8 induces an immediate neutropenia followed by a rebound granulocytosis. In this report, we discuss the effects of IL-8 on the mobilization of hematopoietic stem cells. Within 20 minutes following a single intraperitoneal injection in mice, IL-8 induces the mobilization of hematopoietic progenitor cells (HPC) with colony-forming, radioprotective, and long-term lymphomyeloid resubpopulating ability. Mobilization can be specifically prevented by pretreatment with antibodies against the beta2 integrin LFA-1 (CD11a). In monkeys, IL-8 Induces the rapid release of the metalloproteinase gelatinase-B concurrent with the mobilization of HPC. The latter effect can be prevented by blocking gelatinase-B activity using specific monoclonal antibodies, suggesting the involvement of gelatinase-B as a mediator of HPC mobilization. These results are consistent with the hypothesis that neutrophils are major regulators of stem-cell mobilization through the release of metalloproteinases (MMPs) that cleave extracellular matrix molecules to which HPC are attached.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalSeminars in Hematology
Volume37
Issue number1 Suppl 2
Publication statusPublished - Jan 2000

Cite this

Fibbe, W. E., Pruijt, J. F., van Kooyk, Y., Figdor, C. G., Opdenakker, G., & Willemze, R. (2000). The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization. Seminars in Hematology, 37(1 Suppl 2), 19-24.
Fibbe, W E ; Pruijt, J F ; van Kooyk, Y ; Figdor, C G ; Opdenakker, G ; Willemze, R. / The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization. In: Seminars in Hematology. 2000 ; Vol. 37, No. 1 Suppl 2. pp. 19-24.
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abstract = "The chemokine interleukin-8 (IL-8) is a potent chemoattractant and activator of neutrophils. Upon systemic injection, IL-8 induces an immediate neutropenia followed by a rebound granulocytosis. In this report, we discuss the effects of IL-8 on the mobilization of hematopoietic stem cells. Within 20 minutes following a single intraperitoneal injection in mice, IL-8 induces the mobilization of hematopoietic progenitor cells (HPC) with colony-forming, radioprotective, and long-term lymphomyeloid resubpopulating ability. Mobilization can be specifically prevented by pretreatment with antibodies against the beta2 integrin LFA-1 (CD11a). In monkeys, IL-8 Induces the rapid release of the metalloproteinase gelatinase-B concurrent with the mobilization of HPC. The latter effect can be prevented by blocking gelatinase-B activity using specific monoclonal antibodies, suggesting the involvement of gelatinase-B as a mediator of HPC mobilization. These results are consistent with the hypothesis that neutrophils are major regulators of stem-cell mobilization through the release of metalloproteinases (MMPs) that cleave extracellular matrix molecules to which HPC are attached.",
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author = "Fibbe, {W E} and Pruijt, {J F} and {van Kooyk}, Y and Figdor, {C G} and G Opdenakker and R Willemze",
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Fibbe, WE, Pruijt, JF, van Kooyk, Y, Figdor, CG, Opdenakker, G & Willemze, R 2000, 'The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization' Seminars in Hematology, vol. 37, no. 1 Suppl 2, pp. 19-24.

The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization. / Fibbe, W E; Pruijt, J F; van Kooyk, Y; Figdor, C G; Opdenakker, G; Willemze, R.

In: Seminars in Hematology, Vol. 37, No. 1 Suppl 2, 01.2000, p. 19-24.

Research output: Contribution to journalReview articleAcademicpeer-review

TY - JOUR

T1 - The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization

AU - Fibbe, W E

AU - Pruijt, J F

AU - van Kooyk, Y

AU - Figdor, C G

AU - Opdenakker, G

AU - Willemze, R

PY - 2000/1

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N2 - The chemokine interleukin-8 (IL-8) is a potent chemoattractant and activator of neutrophils. Upon systemic injection, IL-8 induces an immediate neutropenia followed by a rebound granulocytosis. In this report, we discuss the effects of IL-8 on the mobilization of hematopoietic stem cells. Within 20 minutes following a single intraperitoneal injection in mice, IL-8 induces the mobilization of hematopoietic progenitor cells (HPC) with colony-forming, radioprotective, and long-term lymphomyeloid resubpopulating ability. Mobilization can be specifically prevented by pretreatment with antibodies against the beta2 integrin LFA-1 (CD11a). In monkeys, IL-8 Induces the rapid release of the metalloproteinase gelatinase-B concurrent with the mobilization of HPC. The latter effect can be prevented by blocking gelatinase-B activity using specific monoclonal antibodies, suggesting the involvement of gelatinase-B as a mediator of HPC mobilization. These results are consistent with the hypothesis that neutrophils are major regulators of stem-cell mobilization through the release of metalloproteinases (MMPs) that cleave extracellular matrix molecules to which HPC are attached.

AB - The chemokine interleukin-8 (IL-8) is a potent chemoattractant and activator of neutrophils. Upon systemic injection, IL-8 induces an immediate neutropenia followed by a rebound granulocytosis. In this report, we discuss the effects of IL-8 on the mobilization of hematopoietic stem cells. Within 20 minutes following a single intraperitoneal injection in mice, IL-8 induces the mobilization of hematopoietic progenitor cells (HPC) with colony-forming, radioprotective, and long-term lymphomyeloid resubpopulating ability. Mobilization can be specifically prevented by pretreatment with antibodies against the beta2 integrin LFA-1 (CD11a). In monkeys, IL-8 Induces the rapid release of the metalloproteinase gelatinase-B concurrent with the mobilization of HPC. The latter effect can be prevented by blocking gelatinase-B activity using specific monoclonal antibodies, suggesting the involvement of gelatinase-B as a mediator of HPC mobilization. These results are consistent with the hypothesis that neutrophils are major regulators of stem-cell mobilization through the release of metalloproteinases (MMPs) that cleave extracellular matrix molecules to which HPC are attached.

KW - Animals

KW - Cell Adhesion Molecules/physiology

KW - Hematopoietic Stem Cell Mobilization

KW - Humans

KW - Interleukin-8/pharmacology

KW - Matrix Metalloproteinases/drug effects

M3 - Review article

VL - 37

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EP - 24

JO - Seminars in Hematology

JF - Seminars in Hematology

SN - 0037-1963

IS - 1 Suppl 2

ER -

Fibbe WE, Pruijt JF, van Kooyk Y, Figdor CG, Opdenakker G, Willemze R. The role of metalloproteinases and adhesion molecules in interleukin-8-induced stem-cell mobilization. Seminars in Hematology. 2000 Jan;37(1 Suppl 2):19-24.