Tissue-specific expression of IgG receptors by human macrophages ex vivo

Christine W. Bruggeman, Julia Houtzager, Barbara Dierdorp, Jesper Kers, Steven T. Pals, René Lutter, Thomas van Gulik, Joke M. M. den Haan, Timo K. van den Berg, Robin van Bruggen, Taco W. Kuijpers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Recently it was discovered that tissue-resident macrophages derive from embryonic precursors, not only from peripheral blood monocytes, and maintain themselves by self-renewal. Most in-vitro studies on macrophage biology make use of in-vitro cultured human monocytederived macrophages. Phagocytosis of IgG-opsonized particles by tissue-resident macrophages takes place via interaction with IgG receptors, the Fc-gamma receptors (FcγRs). We investigated the FcγR expression on macrophages both in-vivo and ex-vivo from different human tissues. Upon isolation of primary human macrophages from bone marrow, spleen, liver and lung, we observed that macrophages from all studied tissues expressed high levels of FcγRIII, which was in direct contrast with the low expression on blood monocyte- derived macrophages. Expression levels of FcγRI were highly variable, with bone marrow macrophages showing the lowest and alveolar macrophages the highest expression. Kupffer cells in the liver were the only tissue-resident macrophages that expressed the inhibitory IgG receptor, FcγRIIB. This inhibitory receptor was also found to be expressed by sinusoidal endothelial cells in the liver. In sum, our immunofluorescence data combined with ex-vivo stainings of isolated macrophages indicated that tissue-resident macrophages are remarkably unique and different from monocyte-derived macrophages in their phenotypic expression of IgG receptors. Tissue macrophages show distinct tissue-specific FcγR expression patterns.
Original languageEnglish
Article numbere0223264
JournalPLoS ONE
Volume14
Issue number10
DOIs
Publication statusPublished - 1 Jan 2019

Cite this

Bruggeman, C. W., Houtzager, J., Dierdorp, B., Kers, J., Pals, S. T., Lutter, R., ... Kuijpers, T. W. (2019). Tissue-specific expression of IgG receptors by human macrophages ex vivo. PLoS ONE, 14(10), [e0223264]. https://doi.org/10.1371/journal.pone.0223264
Bruggeman, Christine W. ; Houtzager, Julia ; Dierdorp, Barbara ; Kers, Jesper ; Pals, Steven T. ; Lutter, René ; van Gulik, Thomas ; den Haan, Joke M. M. ; van den Berg, Timo K. ; van Bruggen, Robin ; Kuijpers, Taco W. / Tissue-specific expression of IgG receptors by human macrophages ex vivo. In: PLoS ONE. 2019 ; Vol. 14, No. 10.
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abstract = "Recently it was discovered that tissue-resident macrophages derive from embryonic precursors, not only from peripheral blood monocytes, and maintain themselves by self-renewal. Most in-vitro studies on macrophage biology make use of in-vitro cultured human monocytederived macrophages. Phagocytosis of IgG-opsonized particles by tissue-resident macrophages takes place via interaction with IgG receptors, the Fc-gamma receptors (FcγRs). We investigated the FcγR expression on macrophages both in-vivo and ex-vivo from different human tissues. Upon isolation of primary human macrophages from bone marrow, spleen, liver and lung, we observed that macrophages from all studied tissues expressed high levels of FcγRIII, which was in direct contrast with the low expression on blood monocyte- derived macrophages. Expression levels of FcγRI were highly variable, with bone marrow macrophages showing the lowest and alveolar macrophages the highest expression. Kupffer cells in the liver were the only tissue-resident macrophages that expressed the inhibitory IgG receptor, FcγRIIB. This inhibitory receptor was also found to be expressed by sinusoidal endothelial cells in the liver. In sum, our immunofluorescence data combined with ex-vivo stainings of isolated macrophages indicated that tissue-resident macrophages are remarkably unique and different from monocyte-derived macrophages in their phenotypic expression of IgG receptors. Tissue macrophages show distinct tissue-specific FcγR expression patterns.",
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Bruggeman, CW, Houtzager, J, Dierdorp, B, Kers, J, Pals, ST, Lutter, R, van Gulik, T, den Haan, JMM, van den Berg, TK, van Bruggen, R & Kuijpers, TW 2019, 'Tissue-specific expression of IgG receptors by human macrophages ex vivo' PLoS ONE, vol. 14, no. 10, e0223264. https://doi.org/10.1371/journal.pone.0223264

Tissue-specific expression of IgG receptors by human macrophages ex vivo. / Bruggeman, Christine W.; Houtzager, Julia; Dierdorp, Barbara; Kers, Jesper; Pals, Steven T.; Lutter, René; van Gulik, Thomas; den Haan, Joke M. M.; van den Berg, Timo K.; van Bruggen, Robin; Kuijpers, Taco W.

In: PLoS ONE, Vol. 14, No. 10, e0223264, 01.01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Bruggeman, Christine W.

AU - Houtzager, Julia

AU - Dierdorp, Barbara

AU - Kers, Jesper

AU - Pals, Steven T.

AU - Lutter, René

AU - van Gulik, Thomas

AU - den Haan, Joke M. M.

AU - van den Berg, Timo K.

AU - van Bruggen, Robin

AU - Kuijpers, Taco W.

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Bruggeman CW, Houtzager J, Dierdorp B, Kers J, Pals ST, Lutter R et al. Tissue-specific expression of IgG receptors by human macrophages ex vivo. PLoS ONE. 2019 Jan 1;14(10). e0223264. https://doi.org/10.1371/journal.pone.0223264