TNF-α promoter polymorphisms, production and susceptibility to multiple sclerosis in different groups of patients

Tom W.J. Huizinga*, Rudi G.J. Westendorp, Eduard L.E.M. Bollen, Vivian Keijsers, Brigitta M.N. Brinkman, Jan A.M. Langermans, Ferdinand C. Breedveld, Cornelis L. Verweij, Luc De Van Gaer, Lieve Dams, J. Bart A. Crusius, Asunción García-Gonzalez, Bob W. Van Oosten, Chris H. Polman, A. Salvador Peña

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


TNF-α production in whole blood cultures upon stimulation with LPS was determined in 179 individuals from 61 families in order to characterise the magnitude of inherited differences in TNF-α production. The three families characterised by highest TNF production showed 7.1 ± 0.3 ng TNF/ml upon culture with 10 ng LPS and 10.2 ± 0.2 ng TNF/ml upon culture with 1000 ng LPS, in contrast to the three families characterised by the lowest TNF production that showed a production of 1.6 ± 0.1 ng TNF upon culture with 10 ng and 2.5 ± 0.2 ng/ml upon culture with 1000 ng LPS/ml. This difference could not be attributed to the promoter polymorphisms -308 G to A, -238 G to A or -376 G to A, although the -238 GA donors produced 2.1 ± 0.9 ng TNF upon culture with 10 ng endotoxin compared to 3.2 ± 2.2 ng TNF for the -238 GG donors. In line with these results the frequency of the -238 GG genotype was increased in hospitalized MS patients in a nursing home (100% 238GG, n = 57) compared to MS patients in an outpatient's clinic (94% 238GG, n = 98) or Dutch controls (90% 238GG, n = 180). These results suggest that the -238 GG genotype is differently distributed in hospitalized MS patients in a nursing home.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalJournal of Neuroimmunology
Issue number2
Publication statusPublished - 1 Feb 1997

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