Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models

Marielle H. S. Deurloo, Ekaterina Turlova, Wen-Liang Chen, You Wei Lin, Elaine Tam, Nardos G. Tassew, Michael Wu, Ya-Chi Huang, Jacqueline N. Crawley, Philippe P. Monnier, Alexander J. A. Groffen, Hong-Shuo Sun, Lucy R. Osborne, Zhong-Ping Feng

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Williams syndrome (WS) and 7q11.23 duplication syndrome (Dup7q11.23) are neurodevelopmental disorders caused by the deletion and duplication, respectively, of ~ 25 protein-coding genes on chromosome 7q11.23. The general transcription factor 2I (GTF2I, protein TFII-I) is one of these proteins and has been implicated in the neurodevelopmental phenotypes of WS and Dup7q11.23. Here, we investigated the effect of copy number alterations in Gtf2i on neuronal maturation and intracellular calcium entry mechanisms known to be associated with this process. Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. In contrast, mice with 3 copies of Gtf2i (Gtf2i+/Dup) had decreases in axon outgrowth and in TRPC3-mediated calcium entry. The underlying mechanism was that TFII-I did not affect TRPC3 protein expression, while it regulated TRPC3 membrane translocation. Together, our results provide novel functional insight into the cellular mechanisms that underlie neuronal maturation in the context of the 7q11.23 disorders.
Original languageEnglish
JournalMolecular Neurobiology
DOIs
Publication statusE-pub ahead of print - 2018

Cite this

Deurloo, M. H. S., Turlova, E., Chen, W-L., Lin, Y. W., Tam, E., Tassew, N. G., ... Feng, Z-P. (2018). Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models. Molecular Neurobiology. https://doi.org/10.1007/s12035-018-1290-7
Deurloo, Marielle H. S. ; Turlova, Ekaterina ; Chen, Wen-Liang ; Lin, You Wei ; Tam, Elaine ; Tassew, Nardos G. ; Wu, Michael ; Huang, Ya-Chi ; Crawley, Jacqueline N. ; Monnier, Philippe P. ; Groffen, Alexander J. A. ; Sun, Hong-Shuo ; Osborne, Lucy R. ; Feng, Zhong-Ping. / Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models. In: Molecular Neurobiology. 2018.
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title = "Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models",
abstract = "Williams syndrome (WS) and 7q11.23 duplication syndrome (Dup7q11.23) are neurodevelopmental disorders caused by the deletion and duplication, respectively, of ~ 25 protein-coding genes on chromosome 7q11.23. The general transcription factor 2I (GTF2I, protein TFII-I) is one of these proteins and has been implicated in the neurodevelopmental phenotypes of WS and Dup7q11.23. Here, we investigated the effect of copy number alterations in Gtf2i on neuronal maturation and intracellular calcium entry mechanisms known to be associated with this process. Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. In contrast, mice with 3 copies of Gtf2i (Gtf2i+/Dup) had decreases in axon outgrowth and in TRPC3-mediated calcium entry. The underlying mechanism was that TFII-I did not affect TRPC3 protein expression, while it regulated TRPC3 membrane translocation. Together, our results provide novel functional insight into the cellular mechanisms that underlie neuronal maturation in the context of the 7q11.23 disorders.",
author = "Deurloo, {Marielle H. S.} and Ekaterina Turlova and Wen-Liang Chen and Lin, {You Wei} and Elaine Tam and Tassew, {Nardos G.} and Michael Wu and Ya-Chi Huang and Crawley, {Jacqueline N.} and Monnier, {Philippe P.} and Groffen, {Alexander J. A.} and Hong-Shuo Sun and Osborne, {Lucy R.} and Zhong-Ping Feng",
year = "2018",
doi = "10.1007/s12035-018-1290-7",
language = "English",
journal = "Molecular Neurobiology",
issn = "0893-7648",
publisher = "Humana Press",

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Deurloo, MHS, Turlova, E, Chen, W-L, Lin, YW, Tam, E, Tassew, NG, Wu, M, Huang, Y-C, Crawley, JN, Monnier, PP, Groffen, AJA, Sun, H-S, Osborne, LR & Feng, Z-P 2018, 'Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models' Molecular Neurobiology. https://doi.org/10.1007/s12035-018-1290-7

Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models. / Deurloo, Marielle H. S.; Turlova, Ekaterina; Chen, Wen-Liang; Lin, You Wei; Tam, Elaine; Tassew, Nardos G.; Wu, Michael; Huang, Ya-Chi; Crawley, Jacqueline N.; Monnier, Philippe P.; Groffen, Alexander J. A.; Sun, Hong-Shuo; Osborne, Lucy R.; Feng, Zhong-Ping.

In: Molecular Neurobiology, 2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models

AU - Deurloo, Marielle H. S.

AU - Turlova, Ekaterina

AU - Chen, Wen-Liang

AU - Lin, You Wei

AU - Tam, Elaine

AU - Tassew, Nardos G.

AU - Wu, Michael

AU - Huang, Ya-Chi

AU - Crawley, Jacqueline N.

AU - Monnier, Philippe P.

AU - Groffen, Alexander J. A.

AU - Sun, Hong-Shuo

AU - Osborne, Lucy R.

AU - Feng, Zhong-Ping

PY - 2018

Y1 - 2018

N2 - Williams syndrome (WS) and 7q11.23 duplication syndrome (Dup7q11.23) are neurodevelopmental disorders caused by the deletion and duplication, respectively, of ~ 25 protein-coding genes on chromosome 7q11.23. The general transcription factor 2I (GTF2I, protein TFII-I) is one of these proteins and has been implicated in the neurodevelopmental phenotypes of WS and Dup7q11.23. Here, we investigated the effect of copy number alterations in Gtf2i on neuronal maturation and intracellular calcium entry mechanisms known to be associated with this process. Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. In contrast, mice with 3 copies of Gtf2i (Gtf2i+/Dup) had decreases in axon outgrowth and in TRPC3-mediated calcium entry. The underlying mechanism was that TFII-I did not affect TRPC3 protein expression, while it regulated TRPC3 membrane translocation. Together, our results provide novel functional insight into the cellular mechanisms that underlie neuronal maturation in the context of the 7q11.23 disorders.

AB - Williams syndrome (WS) and 7q11.23 duplication syndrome (Dup7q11.23) are neurodevelopmental disorders caused by the deletion and duplication, respectively, of ~ 25 protein-coding genes on chromosome 7q11.23. The general transcription factor 2I (GTF2I, protein TFII-I) is one of these proteins and has been implicated in the neurodevelopmental phenotypes of WS and Dup7q11.23. Here, we investigated the effect of copy number alterations in Gtf2i on neuronal maturation and intracellular calcium entry mechanisms known to be associated with this process. Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. In contrast, mice with 3 copies of Gtf2i (Gtf2i+/Dup) had decreases in axon outgrowth and in TRPC3-mediated calcium entry. The underlying mechanism was that TFII-I did not affect TRPC3 protein expression, while it regulated TRPC3 membrane translocation. Together, our results provide novel functional insight into the cellular mechanisms that underlie neuronal maturation in the context of the 7q11.23 disorders.

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U2 - 10.1007/s12035-018-1290-7

DO - 10.1007/s12035-018-1290-7

M3 - Article

JO - Molecular Neurobiology

JF - Molecular Neurobiology

SN - 0893-7648

ER -