Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway

G Koopman, Y van Kooyk, M de Graaff, C J Meyer, C G Figdor, S T Pals

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The CD44 molecule, a molecule which has been previously known as Hermes, Pgp-1, extracellular matrix receptor III, and In(Lu)-related p80, is currently thought to be involved in several steps of normal immune cell function, including lymphocyte adhesion to high endothelial venules and to the extracellular matrix and T cell activation. We now demonstrate that triggering of CD44 on T lymphocytes by anti-CD44 mAb promotes cell adhesion. The induced homotypic adhesion is mediated by lymphocyte function-associated antigen-1 (LFA-1), because it was inhibited by anti-LFA-1 antibodies and not by anti-LFA-3 antibodies. This notion is supported by the temperature and Mg2+ dependence which is characteristic of LFA-1-mediated adhesion. Moreover, the sensitivity of CD44-induced adhesion to AMG and H7, which both prevent the activation of protein kinase C, and to cytochalasin B, which inhibits microfilament formation, suggests that the activation of the LFA-1 pathway via CD44 involves protein kinase C activation and requires an intact cytoskeleton.

Original languageEnglish
Pages (from-to)3589-93
Number of pages5
JournalJournal of Immunology
Volume145
Issue number11
Publication statusPublished - 1 Dec 1990

Cite this

Koopman, G., van Kooyk, Y., de Graaff, M., Meyer, C. J., Figdor, C. G., & Pals, S. T. (1990). Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway. Journal of Immunology, 145(11), 3589-93.
Koopman, G ; van Kooyk, Y ; de Graaff, M ; Meyer, C J ; Figdor, C G ; Pals, S T. / Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway. In: Journal of Immunology. 1990 ; Vol. 145, No. 11. pp. 3589-93.
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title = "Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway",
abstract = "The CD44 molecule, a molecule which has been previously known as Hermes, Pgp-1, extracellular matrix receptor III, and In(Lu)-related p80, is currently thought to be involved in several steps of normal immune cell function, including lymphocyte adhesion to high endothelial venules and to the extracellular matrix and T cell activation. We now demonstrate that triggering of CD44 on T lymphocytes by anti-CD44 mAb promotes cell adhesion. The induced homotypic adhesion is mediated by lymphocyte function-associated antigen-1 (LFA-1), because it was inhibited by anti-LFA-1 antibodies and not by anti-LFA-3 antibodies. This notion is supported by the temperature and Mg2+ dependence which is characteristic of LFA-1-mediated adhesion. Moreover, the sensitivity of CD44-induced adhesion to AMG and H7, which both prevent the activation of protein kinase C, and to cytochalasin B, which inhibits microfilament formation, suggests that the activation of the LFA-1 pathway via CD44 involves protein kinase C activation and requires an intact cytoskeleton.",
keywords = "Antibodies, Monoclonal/immunology, Cell Adhesion, Cell Aggregation, Cytoskeleton/physiology, Humans, Lymphocyte Activation, Lymphocyte Function-Associated Antigen-1/physiology, Magnesium/pharmacology, Receptors, Lymphocyte Homing/physiology, T-Lymphocytes/immunology",
author = "G Koopman and {van Kooyk}, Y and {de Graaff}, M and Meyer, {C J} and Figdor, {C G} and Pals, {S T}",
year = "1990",
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Koopman, G, van Kooyk, Y, de Graaff, M, Meyer, CJ, Figdor, CG & Pals, ST 1990, 'Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway' Journal of Immunology, vol. 145, no. 11, pp. 3589-93.

Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway. / Koopman, G; van Kooyk, Y; de Graaff, M; Meyer, C J; Figdor, C G; Pals, S T.

In: Journal of Immunology, Vol. 145, No. 11, 01.12.1990, p. 3589-93.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Triggering of the CD44 antigen on T lymphocytes promotes T cell adhesion through the LFA-1 pathway

AU - Koopman, G

AU - van Kooyk, Y

AU - de Graaff, M

AU - Meyer, C J

AU - Figdor, C G

AU - Pals, S T

PY - 1990/12/1

Y1 - 1990/12/1

N2 - The CD44 molecule, a molecule which has been previously known as Hermes, Pgp-1, extracellular matrix receptor III, and In(Lu)-related p80, is currently thought to be involved in several steps of normal immune cell function, including lymphocyte adhesion to high endothelial venules and to the extracellular matrix and T cell activation. We now demonstrate that triggering of CD44 on T lymphocytes by anti-CD44 mAb promotes cell adhesion. The induced homotypic adhesion is mediated by lymphocyte function-associated antigen-1 (LFA-1), because it was inhibited by anti-LFA-1 antibodies and not by anti-LFA-3 antibodies. This notion is supported by the temperature and Mg2+ dependence which is characteristic of LFA-1-mediated adhesion. Moreover, the sensitivity of CD44-induced adhesion to AMG and H7, which both prevent the activation of protein kinase C, and to cytochalasin B, which inhibits microfilament formation, suggests that the activation of the LFA-1 pathway via CD44 involves protein kinase C activation and requires an intact cytoskeleton.

AB - The CD44 molecule, a molecule which has been previously known as Hermes, Pgp-1, extracellular matrix receptor III, and In(Lu)-related p80, is currently thought to be involved in several steps of normal immune cell function, including lymphocyte adhesion to high endothelial venules and to the extracellular matrix and T cell activation. We now demonstrate that triggering of CD44 on T lymphocytes by anti-CD44 mAb promotes cell adhesion. The induced homotypic adhesion is mediated by lymphocyte function-associated antigen-1 (LFA-1), because it was inhibited by anti-LFA-1 antibodies and not by anti-LFA-3 antibodies. This notion is supported by the temperature and Mg2+ dependence which is characteristic of LFA-1-mediated adhesion. Moreover, the sensitivity of CD44-induced adhesion to AMG and H7, which both prevent the activation of protein kinase C, and to cytochalasin B, which inhibits microfilament formation, suggests that the activation of the LFA-1 pathway via CD44 involves protein kinase C activation and requires an intact cytoskeleton.

KW - Antibodies, Monoclonal/immunology

KW - Cell Adhesion

KW - Cell Aggregation

KW - Cytoskeleton/physiology

KW - Humans

KW - Lymphocyte Activation

KW - Lymphocyte Function-Associated Antigen-1/physiology

KW - Magnesium/pharmacology

KW - Receptors, Lymphocyte Homing/physiology

KW - T-Lymphocytes/immunology

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JO - Journal of Immunology

JF - Journal of Immunology

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ER -