Two different mechanisms of immune-complex trapping in the mouse spleen during immune responses

K Yoshida, T K van den Berg, C D Dijkstra

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The capacity of immune-complex (IC) trapping was examined using purified horse radish peroxidase (HRP)-anti-HRP (PAP) on frozen sections of mouse spleen in vitro. We investigated the trapping mechanisms by applying the IC with or without fresh mouse serum added on the spleen sections of naive as well as immunized mice. When the PAP was applied alone, it mainly located on the macrophages in red pulp. In the splenic white pulp of immunized mice, PAP was trapped on follicular dendritic cells (FDC) in a small area of the germinal center whereas it scarcely bound to the splenic white pulp of non-immunized mice. An antibody against mouse Fc receptor (2.4G2) blocked the trapping but antibodies against mouse complement receptor (8C12, Mac-1 and 7G6) did not. When the PAP was applied mixed with fresh mouse serum, it bound on FDC in the primary follicles in the spleen of non-immunized mice. The density and area of IC trapping increased in the spleen of immunized mice. IC trapping in the presence of fresh mouse serum was blocked by the antibodies 8C12 and 7G6 but not by 2.4G2 or Mac-1.

Original languageEnglish
Pages (from-to)377-82
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume329
Publication statusPublished - 1993

Cite this

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title = "Two different mechanisms of immune-complex trapping in the mouse spleen during immune responses",
abstract = "The capacity of immune-complex (IC) trapping was examined using purified horse radish peroxidase (HRP)-anti-HRP (PAP) on frozen sections of mouse spleen in vitro. We investigated the trapping mechanisms by applying the IC with or without fresh mouse serum added on the spleen sections of naive as well as immunized mice. When the PAP was applied alone, it mainly located on the macrophages in red pulp. In the splenic white pulp of immunized mice, PAP was trapped on follicular dendritic cells (FDC) in a small area of the germinal center whereas it scarcely bound to the splenic white pulp of non-immunized mice. An antibody against mouse Fc receptor (2.4G2) blocked the trapping but antibodies against mouse complement receptor (8C12, Mac-1 and 7G6) did not. When the PAP was applied mixed with fresh mouse serum, it bound on FDC in the primary follicles in the spleen of non-immunized mice. The density and area of IC trapping increased in the spleen of immunized mice. IC trapping in the presence of fresh mouse serum was blocked by the antibodies 8C12 and 7G6 but not by 2.4G2 or Mac-1.",
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author = "K Yoshida and {van den Berg}, {T K} and Dijkstra, {C D}",
year = "1993",
language = "English",
volume = "329",
pages = "377--82",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
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Two different mechanisms of immune-complex trapping in the mouse spleen during immune responses. / Yoshida, K; van den Berg, T K; Dijkstra, C D.

In: Advances in Experimental Medicine and Biology, Vol. 329, 1993, p. 377-82.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Two different mechanisms of immune-complex trapping in the mouse spleen during immune responses

AU - Yoshida, K

AU - van den Berg, T K

AU - Dijkstra, C D

PY - 1993

Y1 - 1993

N2 - The capacity of immune-complex (IC) trapping was examined using purified horse radish peroxidase (HRP)-anti-HRP (PAP) on frozen sections of mouse spleen in vitro. We investigated the trapping mechanisms by applying the IC with or without fresh mouse serum added on the spleen sections of naive as well as immunized mice. When the PAP was applied alone, it mainly located on the macrophages in red pulp. In the splenic white pulp of immunized mice, PAP was trapped on follicular dendritic cells (FDC) in a small area of the germinal center whereas it scarcely bound to the splenic white pulp of non-immunized mice. An antibody against mouse Fc receptor (2.4G2) blocked the trapping but antibodies against mouse complement receptor (8C12, Mac-1 and 7G6) did not. When the PAP was applied mixed with fresh mouse serum, it bound on FDC in the primary follicles in the spleen of non-immunized mice. The density and area of IC trapping increased in the spleen of immunized mice. IC trapping in the presence of fresh mouse serum was blocked by the antibodies 8C12 and 7G6 but not by 2.4G2 or Mac-1.

AB - The capacity of immune-complex (IC) trapping was examined using purified horse radish peroxidase (HRP)-anti-HRP (PAP) on frozen sections of mouse spleen in vitro. We investigated the trapping mechanisms by applying the IC with or without fresh mouse serum added on the spleen sections of naive as well as immunized mice. When the PAP was applied alone, it mainly located on the macrophages in red pulp. In the splenic white pulp of immunized mice, PAP was trapped on follicular dendritic cells (FDC) in a small area of the germinal center whereas it scarcely bound to the splenic white pulp of non-immunized mice. An antibody against mouse Fc receptor (2.4G2) blocked the trapping but antibodies against mouse complement receptor (8C12, Mac-1 and 7G6) did not. When the PAP was applied mixed with fresh mouse serum, it bound on FDC in the primary follicles in the spleen of non-immunized mice. The density and area of IC trapping increased in the spleen of immunized mice. IC trapping in the presence of fresh mouse serum was blocked by the antibodies 8C12 and 7G6 but not by 2.4G2 or Mac-1.

KW - Animals

KW - Antigen-Antibody Complex/analysis

KW - Complement System Proteins/immunology

KW - Dendritic Cells/immunology

KW - Female

KW - Immunization

KW - Immunoenzyme Techniques

KW - Macrophages/immunology

KW - Mice

KW - Mice, Inbred C3H/blood

KW - Rats

KW - Receptors, Complement/immunology

KW - Receptors, Fc/immunology

KW - Sheep/blood

KW - Spleen/immunology

M3 - Article

VL - 329

SP - 377

EP - 382

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -