Typing and subtyping of Clostridium difficile isolates by using multiple-locus variable-number tandem-repeat analysis

Renate J. Van Den Berg, Inge Schaap, Kate E. Templeton, Corné H.W. Klaassen, Ed J. Kuijper*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Using the genomic sequence of Clostridium difficile strain 630, we developed multiple-locus variable-number tandem-repeat analysis (MLVA) with automated fragment analysis and multicolored capillary electrophoresis as a typing method for C. difficile. All reference strains, representing 31 serogroups, 25 toxinotypes, and 7 known subtypes of PCR ribotype 001, could be discriminated from each other. Application of MLVA to 28 isolates from 7 outbreaks due to the emerging hyperviralent PCR ribotype 027-pulsed-fieId gel electrophoresis type NAP1 resulted in recognition of 13 clusters. Additionally, 29 toxin A-negative, toxin B-positive isolates belonging to PCR ribotype 017 from eight different countries revealed eight country-specific clusters. MLVA is a highly discriminatory genotyping method and a new tool for subtyping of newly emerging variants of C. difficile.

Original languageEnglish
Pages (from-to)1024-1028
Number of pages5
JournalJournal of Clinical Microbiology
Issue number3
Publication statusPublished - Mar 2007

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