Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Pouline Mp van Oort; Tamara M. Nijsen; Iain R. White; Hugo H. Knobel; Timothy Felton; Nicholas Rattray; Oluwasola Lawal; Murtaza Bulut; Waqar Ahmed; Antonio Artigas; Pedro R. Povoa; Ignacio Martin-Loeches; Hans Weda; Royston Goodacre; Marcus J. Schultz; Paul M. Dark; Stephen J. Fowler; Lieuwe D. Bos

doi:10.1136/thoraxjnl-2021-217362

Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Pouline Mp van Oort, Tamara M. Nijsen, Iain R. White, Hugo H. Knobel, Timothy Felton, Nicholas Rattray, Oluwasola Lawal, Murtaza Bulut, Waqar Ahmed, Antonio Artigas, Pedro R. Povoa, Ignacio Martin-Loeches, Hans Weda, Royston Goodacre, Marcus J. Schultz, Paul M. Dark, Stephen J. Fowler, Lieuwe D. Bos^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

Original language	English
Pages (from-to)	79-81
Journal	Thorax
Volume	77
Issue number	1
DOIs	https://doi.org/10.1136/thoraxjnl-2021-217362
Publication status	Published - 1 Jan 2022

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van Oort, P. M., Nijsen, T. M., White, I. R., Knobel, H. H., Felton, T., Rattray, N., Lawal, O., Bulut, M., Ahmed, W., Artigas, A., Povoa, P. R., Martin-Loeches, I., Weda, H., Goodacre, R., Schultz, M. J., Dark, P. M., Fowler, S. J., & Bos, L. D. (2022). Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx). Thorax, 77(1), 79-81. https://doi.org/10.1136/thoraxjnl-2021-217362

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title = "Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)",

abstract = "Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.",

author = "{van Oort}, {Pouline Mp} and Nijsen, {Tamara M.} and White, {Iain R.} and Knobel, {Hugo H.} and Timothy Felton and Nicholas Rattray and Oluwasola Lawal and Murtaza Bulut and Waqar Ahmed and Antonio Artigas and Povoa, {Pedro R.} and Ignacio Martin-Loeches and Hans Weda and Royston Goodacre and Schultz, {Marcus J.} and Dark, {Paul M.} and Fowler, {Stephen J.} and Bos, {Lieuwe D.}",

year = "2022",

month = jan,

day = "1",

doi = "10.1136/thoraxjnl-2021-217362",

language = "English",

volume = "77",

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journal = "Thorax",

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van Oort, PM, Nijsen, TM, White, IR, Knobel, HH, Felton, T, Rattray, N, Lawal, O, Bulut, M, Ahmed, W, Artigas, A, Povoa, PR, Martin-Loeches, I, Weda, H, Goodacre, R, Schultz, MJ, Dark, PM, Fowler, SJ & Bos, LD 2022, 'Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)', Thorax, vol. 77, no. 1, pp. 79-81. https://doi.org/10.1136/thoraxjnl-2021-217362

TY - JOUR

T1 - Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

AU - van Oort, Pouline Mp

AU - Nijsen, Tamara M.

AU - White, Iain R.

AU - Knobel, Hugo H.

AU - Felton, Timothy

AU - Rattray, Nicholas

AU - Lawal, Oluwasola

AU - Bulut, Murtaza

AU - Ahmed, Waqar

AU - Artigas, Antonio

AU - Povoa, Pedro R.

AU - Martin-Loeches, Ignacio

AU - Weda, Hans

AU - Goodacre, Royston

AU - Schultz, Marcus J.

AU - Dark, Paul M.

AU - Fowler, Stephen J.

AU - Bos, Lieuwe D.

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

AB - Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

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UR - https://www.ncbi.nlm.nih.gov/pubmed/34088787

U2 - 10.1136/thoraxjnl-2021-217362

DO - 10.1136/thoraxjnl-2021-217362

M3 - Article

C2 - 34088787

SN - 0040-6376

VL - 77

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JO - Thorax

JF - Thorax

IS - 1

ER -

Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Abstract

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