Use of N-acetylethyleneimine [AEI] for the inactivation of Semliki Forest virus in vitro

S Amor, H E Webb

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degrees C and in concentration of AEI from 0.025 to 0.1%. The resultant vaccine was inoculated into adult mice to test its antigenicity and into suckling mice to test for the presence of infective virus. Semliki Forest virus-specific IgG was produced equal to that of mice given live virus, and mice were protected against the lethal SFV L10 strain. No suckling mice died, and the brains of the adult mice showed no pathology.

Original languageEnglish
Pages (from-to)367-76
Number of pages10
JournalJournal of Medical Virology
Volume19
Issue number4
Publication statusPublished - Aug 1986

Cite this

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title = "Use of N-acetylethyleneimine [AEI] for the inactivation of Semliki Forest virus in vitro",
abstract = "N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degrees C and in concentration of AEI from 0.025 to 0.1{\%}. The resultant vaccine was inoculated into adult mice to test its antigenicity and into suckling mice to test for the presence of infective virus. Semliki Forest virus-specific IgG was produced equal to that of mice given live virus, and mice were protected against the lethal SFV L10 strain. No suckling mice died, and the brains of the adult mice showed no pathology.",
keywords = "Animals, Antibodies, Viral, Azirines, Cercopithecus aethiops, Chick Embryo, Mice, Neutralization Tests, Semliki forest virus, Temperature, Vaccines, Attenuated, Viral Vaccines, Virus Cultivation, Journal Article, Research Support, Non-U.S. Gov't",
author = "S Amor and Webb, {H E}",
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journal = "Journal of Medical Virology",
issn = "0146-6615",
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Use of N-acetylethyleneimine [AEI] for the inactivation of Semliki Forest virus in vitro. / Amor, S; Webb, H E.

In: Journal of Medical Virology, Vol. 19, No. 4, 08.1986, p. 367-76.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Use of N-acetylethyleneimine [AEI] for the inactivation of Semliki Forest virus in vitro

AU - Amor, S

AU - Webb, H E

PY - 1986/8

Y1 - 1986/8

N2 - N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degrees C and in concentration of AEI from 0.025 to 0.1%. The resultant vaccine was inoculated into adult mice to test its antigenicity and into suckling mice to test for the presence of infective virus. Semliki Forest virus-specific IgG was produced equal to that of mice given live virus, and mice were protected against the lethal SFV L10 strain. No suckling mice died, and the brains of the adult mice showed no pathology.

AB - N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degrees C and in concentration of AEI from 0.025 to 0.1%. The resultant vaccine was inoculated into adult mice to test its antigenicity and into suckling mice to test for the presence of infective virus. Semliki Forest virus-specific IgG was produced equal to that of mice given live virus, and mice were protected against the lethal SFV L10 strain. No suckling mice died, and the brains of the adult mice showed no pathology.

KW - Animals

KW - Antibodies, Viral

KW - Azirines

KW - Cercopithecus aethiops

KW - Chick Embryo

KW - Mice

KW - Neutralization Tests

KW - Semliki forest virus

KW - Temperature

KW - Vaccines, Attenuated

KW - Viral Vaccines

KW - Virus Cultivation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 19

SP - 367

EP - 376

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 4

ER -