Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

Jan W. Buikema, Soah Lee, William R. Goodyer, Renee G. Maas, Orlando Chirikian, Guang Li, Yi Miao, Sharon L. Paige, Daniel Lee, Haodi Wu, David T. Paik, Siyeon Rhee, Lei Tian, Francisco X. Galdos, Nazan Puluca, Benjamin Beyersdorf, James Hu, Aimee Beck, Sneha Venkamatran, Srilatha SwamiPaul Wijnker, Maike Schuldt, Larissa M. Dorsch, Alain van Mil, Kristy Red-Horse, Joy Y. Wu, Caroline Geisen, Michael Hesse, Vahid Serpooshan, Stefan Jovinge, Bernd K. Fleischmann, Pieter A. Doevendans, Jolanda van der Velden, K. Christopher Garcia, Joseph C. Wu, Joost P.G. Sluijter, Sean M. Wu*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.

Original languageEnglish
Pages (from-to)50-63
JournalCell Stem Cell
Issue number1
Publication statusPublished - 2 Jul 2020

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